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Study summary

Title	A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.
Abstract	Spermatogenesis requires intricate interactions between the germline and somatic cells. Within a given cross section of a seminiferous tubule, multiple germ and somatic cell types co-occur. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. To address this challenge, we collected single-cell RNA sequencing data from ∼35,000 cells from the adult mouse testis and identified all known germ and somatic cells, as well as two unexpected somatic cell types. Our analysis revealed a continuous developmental trajectory of germ cells from spermatogonia to spermatids and identified candidate transcriptional regulators at several transition points during differentiation. Focused analyses delineated four subtypes of spermatogonia and nine subtypes of Sertoli cells; the latter linked to histologically defined developmental stages over the seminiferous epithelial cycle. Overall, this high-resolution cellular atlas represents a community resource and foundation of knowledge to study germ cell development and in vivo gametogenesis.
Publication date	09. 2018

Dataset summary

Name	data genelevel Adult germ cell lineage Waterfall FigS2C left
Number of sample/cell	20238
Number of classification	2
Number of Interogated genes	28818
Technology	scRNA-Seq
Species	Mus musculus

Classification parameters

Dataset summary

Name	data genelevel Adult germ cell lineage Waterfall FigS2C left
Number of samples/cells	20238
Number of classification	2
Number of interrogated genes	28818
Technology	scRNA-Seq
Species	Mus musculus

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data genelevel Adult germ cell lineage Waterfall FigS2C left - Green et al. 2018, Dev. Cell