Pubmed ID : 26836306
Article Name : Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation.
Abstract : Upon fertilization, the highly specialised sperm and oocyte genomes are remodelled to confer totipotency. The mechanisms of the dramatic reprogramming events that occur have remained unknown, and presumed roles of histone modifying enzymes are just starting to be elucidated. Here, we explore the function of the oocyte-inherited pool of a histone H3K4 and K9 demethylase, LSD1/KDM1A during early mouse development. KDM1A deficiency results in developmental arrest by the two-cell stage, accompanied by dramatic and stepwise alterations in H3K9 and H3K4 methylation patterns. At the transcriptional level, the switch of the maternal-to-zygotic transition fails to be induced properly and LINE-1 retrotransposons are not properly silenced. We propose that KDM1A plays critical roles in establishing the correct epigenetic landscape of the zygote upon fertilization, in preserving genome integrity and in initiating new patterns of genome expression that drive early mouse development.
Publication data : Feb. 2016
Authors : K Ancelin, L Syx, M Borensztein, N Ranisavljevic, I Vassilev, L Briseño-Roa, T Liu, E Metzger, N Servant, E Barillot, CJ Chen, R Schüle, E Heard
Ome : Transcriptome
Technologies : RNA-Seq
Species : Mus musculus (Genome browser )
Experimental design : Mutant
Topics : Oogenesis, Development, Embryogenesis
Tissues : Oocytes, Embryo
Sex : Female
Developmental stage : Fetal
Age : 2cells
Keywords : chromosomes, developmental biology, epigenetic reprogramming, genes, histone demethylation, lysine methylation, mouse, preimplantation development, retrotransposons, stem cells, transcription regulation
Sample count : 30