Pubmed ID : 26836306

Article Name : Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation.

Abstract : Upon fertilization, the highly specialised sperm and oocyte genomes are remodelled to confer totipotency. The mechanisms of the dramatic reprogramming events that occur have remained unknown, and presumed roles of histone modifying enzymes are just starting to be elucidated. Here, we explore the function of the oocyte-inherited pool of a histone H3K4 and K9 demethylase, LSD1/KDM1A during early mouse development. KDM1A deficiency results in developmental arrest by the two-cell stage, accompanied by dramatic and stepwise alterations in H3K9 and H3K4 methylation patterns. At the transcriptional level, the switch of the maternal-to-zygotic transition fails to be induced properly and LINE-1 retrotransposons are not properly silenced. We propose that KDM1A plays critical roles in establishing the correct epigenetic landscape of the zygote upon fertilization, in preserving genome integrity and in initiating new patterns of genome expression that drive early mouse development.

Publication data : Feb. 2016

Authors : K Ancelin, L Syx, M Borensztein, N Ranisavljevic, I Vassilev, L Briseño-Roa, T Liu, E Metzger, N Servant, E Barillot, CJ Chen, R Schüle, E Heard

Ome : Transcriptome

Technologies : RNA-Seq

Species : Mus musculus (Genome browser )

Experimental design : Mutant

Topics : Oogenesis, Development, Embryogenesis

Tissues : Oocytes, Embryo

Sex : Female

Developmental stage : Fetal

Age : 2cells

Keywords : chromosomes, developmental biology, epigenetic reprogramming, genes, histone demethylation, lysine methylation, mouse, preimplantation development, retrotransposons, stem cells, transcription regulation

Sample count : 30


Name Specie Technology Actions
data_genelevel Mus musculus (Genome browser ) RNA-Seq View Download