Pubmed ID : 30053582

Article Name : Transcriptomic analysis of mRNA expression and alternative splicing during mouse sex determination.

Abstract : Mammalian sex determination hinges on sexually dimorphic transcriptional programs in developing fetal gonads. A comprehensive view of these programs is crucial for understanding the normal development of fetal testes and ovaries and the etiology of human disorders of sex development (DSDs), many of which remain unexplained. Using strand-specific RNA-sequencing, we characterized the mouse fetal gonadal transcriptome from 10.5 to 13.5 days post coitum, a key time window in sex determination and gonad development. Our dataset benefits from a greater sensitivity, accuracy and dynamic range compared to microarray studies, allows global dynamics and sex-specificity of gene expression to be assessed, and provides a window to non-transcriptional events such as alternative splicing. Spliceomic analysis uncovered female-specific regulation of Lef1 splicing, which may contribute to the enhanced WNT signaling activity in XX gonads. We provide a user-friendly visualization tool for the complete transcriptomic and spliceomic dataset as a resource for the field.

Publication data : 12. 2018

Authors : L Zhao, C Wang, ML Lehman, M He, J An, T Svingen, CM Spiller, ET Ng, CC Nelson, P Koopman

Ome : Transcriptome

Technologies : RNA-Seq

Species : Mus musculus

Experimental design : Normal

Topics : Gonad development, Sex determination

Tissues : Ovary, Testis

Sex : Female, Male

Developmental stage : Fetal

Age : 10.5dpc, 11.5dpc, 12.5dpc, 13.5dpc

Keywords : Gonadal development, Lef1, Ovary, RNA-Seq, Testis, Transcriptional regulation

Sample count : 16


Name Species Technology Actions
data_genelevel Mus musculus RNA-Seq View Download

Jbrowse datasets(s)

Species Jbrowse
Mus musculus Genome browser