Pubmed ID : 30061742

Article Name : Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis.

Abstract : A systematic interrogation of male germ cells is key to complete understanding of molecular mechanisms governing spermatogenesis and the development of new strategies for infertility therapies and male contraception. Here we develop an approach to purify all types of homogeneous spermatogenic cells by combining transgenic labeling and synchronization of the cycle of the seminiferous epithelium, and subsequent single-cell RNA-sequencing. We reveal extensive and previously uncharacterized dynamic processes and molecular signatures in gene expression, as well as specific patterns of alternative splicing, and novel regulators for specific stages of male germ cell development. Our transcriptomics analyses led us to discover discriminative markers for isolating round spermatids at specific stages, and different embryo developmental potentials between early and late stage spermatids, providing evidence that maturation of round spermatids impacts on embryo development. This work provides valuable insights into mammalian spermatogenesis, and a comprehensive resource for future studies towards the complete elucidation of gametogenesis.

Publication data : 09. 2018

Authors : Y Chen, Y Zheng, Y Gao, Z Lin, S Yang, T Wang, Q Wang, N Xie, R Hua, M Liu, J Sha, MD Griswold, J Li, F Tang, MH Tong

Ome : Transcriptome

Technologies : RNA-Seq

Species : Mus musculus

Experimental design : Normal

Topics : Spermatogenesis

Tissues : Spermatogonia, Spermatogonia and spermatocytes, Spermatocytes, Spermatocytes and round spermatids, Round spermatids

Sex : Male

Developmental stage : Adult

Sample count : 102


Name Species Technology Actions
data_genelevel Mus musculus RNA-Seq View Download

Jbrowse datasets(s)

Species Jbrowse
Mus musculus Genome browser