Pubmed ID : 30146481
Article Name : A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.
Abstract : Spermatogenesis requires intricate interactions between the germline and somatic cells. Within a given cross section of a seminiferous tubule, multiple germ and somatic cell types co-occur. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. To address this challenge, we collected single-cell RNA sequencing data from ∼35,000 cells from the adult mouse testis and identified all known germ and somatic cells, as well as two unexpected somatic cell types. Our analysis revealed a continuous developmental trajectory of germ cells from spermatogonia to spermatids and identified candidate transcriptional regulators at several transition points during differentiation. Focused analyses delineated four subtypes of spermatogonia and nine subtypes of Sertoli cells; the latter linked to histologically defined developmental stages over the seminiferous epithelial cycle. Overall, this high-resolution cellular atlas represents a community resource and foundation of knowledge to study germ cell development and in vivo gametogenesis.
Publication data : 09. 2018
Authors : CD Green, Q Ma, GL Manske, AN Shami, X Zheng, S Marini, L Moritz, C Sultan, SJ Gurczynski, BB Moore, MD Tallquist, JZ Li, SS Hammoud
Ome : Transcriptome
Technologies : scRNA-Seq
Species : Mus musculus
Experimental design : Normal
Topics : Spermatogenesis
Tissues : Testis
Sex : Male
Developmental stage : Adult
Age : 7wpp, 9wpp, 8wpp, 16wpp, 10wpp, 7.5wpp
Sample count : 18
Document(s)
| Name | Species | Technology | Actions |
|---|---|---|---|
| data genelevel Spermatogonia cells Fig4A | Mus musculus | scRNA-Seq | View Download |
| data genelevel Adult germ cell lineage Monocle FigS2C right | Mus musculus | scRNA-Seq | View Download |
| data genelevel Testicular cells Fig1B left | Mus musculus | scRNA-Seq | View Download |
| data genelevel Adult germ cells Fig2A | Mus musculus | scRNA-Seq | View Download |
| data genelevel Somatic cells Fig1B right | Mus musculus | scRNA-Seq | View Download |
| data genelevel Adult germ cell lineage Waterfall FigS2C left | Mus musculus | scRNA-Seq | View Download |
| data genelevel Sertoli cells Fig6B | Mus musculus | scRNA-Seq | View Download |
| data genelevel Somatic cells Fig5A | Mus musculus | scRNA-Seq | View Download |
Jbrowse datasets(s)
No jbrowse datasets are associated with this study