Pubmed ID : 30404016
Article Name : The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
Abstract : Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduction and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for aggregates of certain spermatogenic cell types, the full continuum of gene expression patterns underlying ongoing spermatogenesis in steady state was previously unclear. Here, we catalog single-cell transcriptomes for >62,000 individual spermatogenic cells from immature (postnatal day 6) and adult male mice and adult men. This allowed us to resolve SSC and progenitor spermatogonia, elucidate the full range of gene expression changes during male meiosis and spermiogenesis, and derive unique gene expression signatures for multiple mouse and human spermatogenic cell types and/or subtypes. These transcriptome datasets provide an information-rich resource for studies of SSCs, male meiosis, testicular cancer, male infertility, or contraceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve spermatogenesis in vitro.
Publication data : 11. 2018
Authors : BP Hermann, K Cheng, A Singh, L Roa-De La Cruz, KN Mutoji, IC Chen, H Gildersleeve, JD Lehle, M Mayo, B Westernströer, NC Law, MJ Oatley, EK Velte, BA Niedenberger, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey
Ome : Transcriptome
Technologies : scRNA-Seq
Species : Mus musculus
Experimental design : Normal
Topics : Spermatogenesis
Tissues : Spermatogonia, Spermatogenic cells, Spermatocytes, Spermatids
Sex : Male
Developmental stage : Prepubertal, Adult
Age : 6dpp
Keywords : male germ cells, RNA, heterogeneity, meiosis, spermiogenesis, continuum, human, mouse
Sample count : 589
Document(s)
| Name | Species | Technology | Actions |
|---|---|---|---|
| data genelevel Fig2G adult mouse spermatogonia | Mus musculus | scRNA-Seq | View Download |
| data genelevel Fig4A unselected spermatocytes | Mus musculus | scRNA-Seq | View Download |
| data genelevel Fig6C sorted P6 spermatogonia | Mus musculus | scRNA-Seq | View Download |
| data genelevel Fig2A unselected spermatogonia | Mus musculus | scRNA-Seq | View Download |
| data genelevel FigS5A staput spermatids | Mus musculus | scRNA-Seq | View Download |
| data genelevel FigS4A staput spermatocytes | Mus musculus | scRNA-Seq | View Download |
| data genelevel Fig5A unselected spermatids | Mus musculus | scRNA-Seq | View Download |
| data genelevel Fig6A unselected P6 spermatogonia | Mus musculus | scRNA-Seq | View Download |
| data genelevel Fig1A steady spermatogenesis | Mus musculus | scRNA-Seq | View Download |
Jbrowse datasets(s)
No jbrowse datasets are associated with this study